Morphometric Subtyping for a Panel of Breast Cancer Cell Lines
IEEE Int. Symp. on Biomedical Imaging-from nano to macro , 2009
A panel of cell lines of diverse molecular background offers
an improved model system for high-content screening, comparative analysis, and
cell systems biology.
pipeline has been developed to collect images from cell-based
assays, segment individual cells and colonies, represent segmented objects in a
multidimensional space, and cluster them
for identifying distinct subpopulations. While each segmentation strategy can vary for different imaging assays, representation and subpopulation analysis share a common thread.
Application of this pipeline to a library of 41 breast cancer
cell lines is demonstrated. These cell lines are grown in 2D
and imaged through immunofluorescence microscopy. Subpopulations in this panel are identified and shown to correlate
with previous subtyping literature that was derived from transcript data.
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